To quote the MAME release notes:
MAME 0.229
24 Feb 2021
It’s been an eventful month, culminating in the release of MAME 0.229 today. One change that you’ll notice straight away is that the “64” suffix is no longer added to the file name for 64-bit versions of MAME. If you’re unsure, you can see the data model at the end of the window title.
in other words: You're pointing to an exe which does not exist anymore. So simply click your profile, update the filename for MAME and load....
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